Fast DNA Library Prep Kit for MGI V2
【Product Name】
Fast DNA Library Prep Kit for MGI V2
【Cat. No./Spec.】
KM001S-A/24 rxns; KM001S-B/96 rxns; sample sack/ 6 rxns
【Product Description】
Aiming at MGI high-throughput sequencing platform, this kit provides a convenient and universal DNA library construction scheme in one tube. It combines end repair and A-Tailing into one step, greatly shortening the time of library construction and reducing the error caused by tedious steps. The end preparation, adapter ligation, amplification and purification of the fragmented double-stranded DNA can be performed within about 2 hours. Complete library quantification can be performed by dsDNA fluorescent dye method (e.g., Thermo Qubit Flex Fluorometer) or absolute quantification PCR after diluting the library to an appropriate concentration.
【Sample Type】
| Application | Sample Type | Recommended Amount |
| Whole genome sequencing | High quality complex genomes | 50ng-1μg |
| Target capture sequencing of whole exome | High quality complex genomes | 10ng-1μg |
| Target capture sequencing of whole genome | FFPE DNA | ≥50ng |
| Target capture sequencing of whole genome | cfDNA/ctDNA | ≥100pg |
| Whole genome sequencing | Microbial genome | 1ng-1μg |
| ChIP-Seq | ChIP DNA | ≥100pg |
| Targeted sequencing | Amplicon | ≥100pg |
【Storage Condition & Shelf Life】
All reagents should be stored at -20°C. Ligation Buffer is normal for crystals to precipitate at low temperatures, it should be balanced to room temperature before use. The product is valid for 12 months.
【Components】
| Component | 24 rxns | 96 rxns |
| End Repair & A-Tailing Enzyme Mix | 120 μl | 2×240 μl |
| End Repair & A-Tailing Buffer | 240 μl | 2×480 μl |
| Fast DNA Ligase | 120 μl | 2×240 μl |
| Fast Ligation Buffer | 600 μl | 4×600 μl |
| 2× HIFI Library PCR Master Mix | 600 μl | 4×600 μl |
| Primer Mix for MGI* | 120 μl | 480 μl |
* If there are more than one sample, #KM002 and #KM003 adapter primer mix is recommended. This kit provides a set of primers, the primer sequence is as follows:
5’-TGTGAGCCAAGGAGTTG-3’
5’-GAACGACATGGCTACGA-3’
Note:recommended selection beads: #NC1011 GDSPure DNA Selection Magbeads or AMPure XP beads.
GDSBio Is a high-tech enterprise focusing on research and development, production and sales of high-quality life science products. The company has a complete product line, with PCR technology as the core, focusing on general PCR, fluorescence quantitative PCR, NGS storage, nucleic acid electrophoresis and other molecular biology technologies, and has developed molecular research reagents, molecular in vitro diagnostic raw materials, nucleic acid extraction and detection reagents and other products.
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