6× Gel Loading Dye, Orange
Item No.: M9071 Size: 1 ml × 5
Description
6× Gel Loading Dye, Orange is a pre-mixed loading buffer with two tracking dyes for agarose and non-denaturing polyacrylamide gel electrophoresis. EDTA is included to chelate magnesium (up to 10mM) in enzymatic reactions, thereby stopping the reaction. Xylene cyanol and Orange G are the standard tracking dyes for electrophoresis.
Contents
10mM Tris-HCl (pH 7.6), 0.03% Xylene Cyanol, 0.15% Orange G,60% Glycerol, 60mM EDTA.
Application
Analysis of large DNA molecules. Preparation of DNA ladders, markers and samples for loading on agarose or polyacrylamide gels.
Features
Two-color tracking of DNA migration during electrophoresis.
No DNA masking during gel exposure to UV light.
EDTA binds divalent metal ions and inhibits metal dependent nucleases.
Storage
Store at RT or at 4 ̊C for up to 12 months. For longer periods, store at -20 ̊C.
Quality Assurance Statement
Gel Loading Dye, Orange (6×) is assayed for absence of endonuclease, exonuclease and no RNase activity.
Usage Recommendations
1.Add 1 volume of 6× Gel Loading Dye, Orange to 5 volumes of DNA sample.
2.Mix well, spin down and load.
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Orange 6x DNA Loading Dye 1mlx5 DNA Electrophoresis Gel Loading Buffer Images
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