5X Reverse Transcription Mixture PowerScript RT SuperMix

PowerScript RT SuperMix

For research use only

Components

Storage

This reagent should be kept at -20°C and is valid for 2 years.

Description

PowerScript RT SuperMix is an optimized 5X reverse transcription mixture that contains all the ingredients required for first strand cDNA synthesis in a two-step RT-qPCR workflow. It contains thermostable reverse transcriptase, which supports cDNA synthesis at high temperatures. It also contains murine-derived RNase inhibitors to protect the template RNA from degradation. The mixture contains random haxmers and Oligo dT primers, which can fully perform reverse transcription of various kinds of RNA. This product can reverse transcribe as low as a single-copy of RNA, and the resulting cDNA product is an ideal template for qPCR detection, ensuring the sensitivity and accuracy of detection.

Application

First strand cDNA synthesis.

Matters Needing Attention

• Successful cDNA synthesis comes from high quality RNA. High quality RNA should at least guarantee full length integrity and contain no inhibitors of reverse transcriptase, such as EDTA or SDS.
• The amount of RNA required for detection depends on the abundance of the transcript of interest. In general, the recommended total RNA is 1 ng to 1 μg or mRNA is 0.1 ng to 100 μg.
• During the operation, care should be taken to prevent RNA degradation due to the introduction of RNase. It is best to store RNA at -70°C and avoid repeated freezing and thawing.
• The presence of genomic DNA or excess product can interfere with the accurate quantification of target RNA, especially for low-copy targets. The No RT control reaction can be simulated by thermal inactivation of PowerScript RT SuperMix at 95°C for 1 minute and then adding template RNA. In addition, an NTC (no template control) reaction should be set up to demonstrate that the reverse transcription is meaningful.
• cDNA products should be stored at or below -20°C and avoid repeated freeze-thaw.

GDSBio has a complete product line. With PCR technology as the core, focusing on molecular biology techniques such as ordinary PCR, fluorescence quantitative PCR, nucleic acid electrophoresis, nucleic acid purification, it has developed reagents for molecular biology research and diagnosis, and completed the layout of the whole industrial chain from molecular biology reagents, medical lab supplies to specialized services.